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論著名稱: 柬埔寨餘甘子萃取物抗發炎效果之研究(Anti-inflammatory Effect Extract of Emblica Fruit from Cambodia)
編著譯者: 林文宏郭恆宏林穎志邱語溱劉大維
出版日期: 2022.04
刊登出處: 台灣/生物產業科技管理叢刊第 10 卷 /26-38 頁
頁  數: 12 點閱次數: 16
下載點數: 48 點 銷售明細: 權利金查詢 變更售價
授 權 者: 財團法人全球生物產業科技發展基金會 授權者指定不分配權利金給作者)
關 鍵 詞: 餘甘子發炎因子巨噬細胞
中文摘要: 本文以柬埔寨餘甘子(Phyllanthus emblica L.)萃取物為材料,研究其對巨噬細胞株的抗發炎效果。應用體外培養的細胞株,以三種促炎因子,包含一氧化氮(nitric oxide, NO)、白介素 6(interleukin 6, IL-6)、腫瘤壞死因子-α(tumor necrosis factor-alpha, TNF-α)進行分析,並進行細胞毒性(microculture tetrazolium assay, MTT assay)分析。結果顯示,柬埔寨餘甘子萃取物具顯著抑制由細菌脂多醣(lipopolysaccharide, LPS)所誘導巨噬細胞株產生大量的 NO 與 IL-6 之發炎反應。對於 NO,在細胞培養液中添加 5% 萃取物的發炎反應抑制率為 31.9%(正控制組之 68.1% ± 5.1%),添加 10% 萃取物的抑制率為 45.8%(正控制組之 54.2% ± 5.6%)。對於 IL-6,添加 5% 的抑制率為 33.9%(正控制組之 66.1% ± 9.7%),添加 10% 的抑制率為 44.0%(正控制組之 56.0% ± 1.3%)。但對 TNF-α 則無顯著抑制作用。同時,在本研究之測試濃度下未測得顯著細胞毒性。綜上所述,柬埔寨餘甘子萃取物在體外培養的細胞模式中,能有效抑制 LPS 所刺激誘導 NO 與 IL-6 的發炎反應。
英文關鍵詞: emblica fruit (Phyllanthus emblica L.)inflammatory factorsmacrophage
英文摘要: This study used emblica fruit (Phyllanthus emblica L.) from Cambodia solution as the material to explore its anti-inflammatory response effect on cells. Using cell lines cultured in vitro to analyze nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) that cause inflammation and processed cytotoxicity (microculture tetrazolium assay, MTT assay) analysis. The results showed that the micronized emblica solution significantly inhibited the inflammatory response of NO and IL-6 induced by the bacterial lipopolysaccharide (LPS) activated macrophage cell line. For NO, the inhibition rate of adding 5% was 31.9% (68.1% ± 5.1%), and the inhibition rate of adding 10% was 45.8% (54.2% ± 5.6%). For IL-6, the inhibition rate of adding 5% was 33.9% (66.1% ± 9.7%), and the inhibition rate of adding 10% was 44.0% (56.0% ± 1.3%). But it has no inhibitory effect on TNF-α. Meanwhile, no cytotoxicity was detected at the tested concentrations. In summary, the micronized emblica solution has effectively inhibited the inflammatory response of NO and IL-6 mediated by LPS stimulation in cells cultured in vitro.
目  次: 壹、前言
貳、材料和研究方法
參、研究結果
肆、結論與建議
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